Co-translational protein insertion into the bacterial plasma membrane
Integral membrane proteins enter the bacterial plasma membrane in a co-translational fashion through the lateral gate of the SecYEG translocon, a protein-conducting channel in the plasma membrane. This project addresses (i) the dynamics of lateral gate opening, (ii) the mechanism of transmembrane segment insertion, in particular of topological inversions, and (iii) the influence of accessory membrane proteins, such as YidC, on the dynamics of the SecYEG translocon. We will apply biochemical and fluorescence-based biophysical methods, including PET, FCS, FRET, and single-molecule FRET.
Prof. Dr. Marina Rodnina
Max Planck Institute for Biophysical Chemistry
Am Faßberg 11
+49 551 201 2901 (phone)